Purification and properties of L-threonine dehydrase of sheep liver.
نویسندگان
چکیده
Materials-L-Threonine, L-serine, and pyridosal-P monohydrate were purchased from the California Corporation for Biochemical Research, a-ketobutyric acid from the Nutritional Biochemical Corporation, pyruvic acid from the Fisher Scientific Company, and DESE-cellulose from Eastman Organic Chemicals. nn-Allothreonine and L-allothreonine were kindly furnished by Dr. Alton iLleister of Tufts University School of Ivleditine.
منابع مشابه
Studies on the nature, inducibility, and assay of the threonine and serine dehydrase activities of rat liver.
Threonine dehydrase (deaminase), which catalyzes the conversion of I,-threonine to a-ketobutyrate and ammonia, has been observed in crude extracts prepared from a number of different organisms (1-13). Frequently these same extracts also act on L-serine to form pyruvate and ammonia. Table I summarizes the available data on the serine and threonine dehydrasel activities in crude extracts from var...
متن کاملPurification and properties of serine and threonine dehydrases.
The work reported herein describes the results of endeavors to isolate and characterize the enzymes involved in the dehydrative deamination of the /?-hydroxyamino acids, serine and threonine. Preparations have been obtained which act on threonine and serine separately. L-Threonine dehydrase and L-serine dehydrase were separated by controlled heat denaturation and subsequent ammonium sulfate fra...
متن کاملSubstrate induction of threonine dehydrase in vivo and in perfused rat livers.
The activity of the enzyme threonine dehydrase in the livers of mice and rats is increased by the intraperitoneal injection of threonine, while that of serine dehydrase shows no change. The injection of a comparable amount of L-serine produces no increase in the activity of either L-serine dehydrase or L-threonine dehydrase. This is substantiating evidence that these two dehydrases are separate...
متن کاملDEAMINATION OF SERINE II. D-SERINE DEHYDRASE, A VITAMIN Bs ENZYME FROM ESCHERICHIA COLP
Non-enzymatic deamination of serine and cysteine is catalyzed by pyridoxal and certain metal salts at 100” (2). This finding suggested that pyridoxal phosphate might be involved in the enzymatic deamination of these amino acids. Vitamin B, has already been implicated in the desulfhydration of cysteine by rat liver (3) and of cysteine and homocysteine by bacteria (4). Several similarities of cys...
متن کاملStudies on the Induction and Repression of Enzymes in Rat Liver III. INDUCTION OF ORNITHINE 6-TRANSAMINASE AND THREONINE DEHYDRASE BY ORAL INTUBATION OF FREE AMINO ACIDS* CARL PERAINO, ROBERT
It is well known that the feeding ad libitum of high protein diets to rats over a period varying from several days to several weeks produces an increase in the activities of enzymes involved in amino acid catabolism (3-8). Studies in our laboratory have shown that at least two amino -acid-catabolizing enzymes, threonine dehydrase and ornithine transaminase, can be elevated to high levels within...
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عنوان ژورنال:
- The Journal of biological chemistry
دوره 236 شماره
صفحات -
تاریخ انتشار 1961